A laboratory researcher handling a sample with a pipette.
Laboratory research scene.
Photo: National Cancer Institute / Unsplash
03 / Sample collection

Urine Sample
Collection

A clear procedure from notification to sealing and analysis. Know who conducts testing, what a suitable sample requires and what to expect throughout collection.

In-competitionOut-of-competition
Authorised testing

Who can authorise a test?

Testing is conducted by authorised personnel on behalf of an organisation with testing authority.

NADO

National Anti-Doping Organisations

IF

International Federations

MEO

Major Event Organisations

TA / Testing Authority
Authorises and directs testing.
SCA / Sample Collection Authority
Arranges sample collection.
RMA / Results Management Authority
Manages results and subsequent procedures.
Sample requirements
90mL

Minimum urine volume

The officer checks both the volume and concentration of the sample.

Specific gravity requirements

The following thresholds apply when measured with a refractometer.

Urine volumeMinimum specific gravity
90 mL to less than 150 mL≥ 1.005
150 mL or more≥ 1.003

For a volume of 90 mL to less than 150 mL, laboratory strips require a reading of at least 1.010. The 1.003 threshold for 150 mL or more applies to refractometer readings only.

From notification to analysis

The testing process

Nine stages explain how the sample is collected, secured and sent for analysis.

  1. Step 01

    Doping control

    Testing takes place under an authorised anti-doping programme, in-competition or out-of-competition.

  2. Step 02

    Athlete selection

    You are notified of selection, shown official identification and informed of the required sample. Acknowledge the notification as instructed.

  3. Step 03

    Report to the station

    Report immediately to the Doping Control Station unless a permitted delay is approved. Remain under observation.

  4. Step 04

    Choose the equipment

    Select a collection vessel. Check that the packaging is sealed and intact, and raise any concern with the officer.

  5. Step 05

    Provide the sample

    Provide your first urine after notification under direct observation by an authorised witness of the same gender.

  6. Step 06

    Split the sample

    Check the A and B kit codes, then divide the sample as directed. Leave a small amount for the officer's specific gravity check.

  7. Step 07

    Seal the samples

    Seal the A and B bottles as instructed. The officer checks that they are securely closed.

  8. Step 08

    Complete the form

    Review the Doping Control Form, record any comments and sign it. Keep your copy or digital record.

  9. Step 09

    The analysis

    The sealed, coded sample is securely transported to a WADA-accredited laboratory for analysis.

Gloved hands using a pipette in a laboratory.
Laboratory testing scene.
Photo: CDC / Unsplash
From station to laboratory

Your sample stays identifiable by code.

Sample codes connect the sealed bottles to the collection record. Documentation sent to the laboratory does not include information identifying you.

Your part in the process

Your rights & responsibilities

Ask for clarification whenever you need it. You should understand the procedure throughout collection.

Your rights

  1. See the Doping Control Officer's identification.
  2. Ask for additional information about sample collection.
  3. Know the authority under which your sample is collected.
  4. Be informed of the type of sample required.
  5. Drink fluids, while following guidance to avoid excessive hydration.
  6. Have a representative present.
  7. Have an interpreter, if available.
  8. Request appropriate modifications if you have an impairment.
  9. Be informed of your rights and responsibilities.
  10. Document any concerns about the process.
  11. Receive a copy or digital record of the collection session.
  12. Request a delay in reporting for valid reasons, subject to approval.

Your responsibilities

  1. Remain within direct observation of the DCO or chaperone.
  2. Provide the first urine passed after notification for collection.
  3. Produce appropriate identification.
  4. Report immediately, unless a permitted delay has been approved.
  5. Follow the DCO or chaperone's directions and collection procedures.

If you have a concern, ask the DCO to explain it and record your comments on the Doping Control Form.

Requesting a delay

A request needs approval.

You must stay under continuous observation. Any approved delay and its reason are documented by the DCO.

IC: In-competition
OOC: Out-of-competition

Reason for the requestICOOC
Performing a warm-downListedNot listed
Competing in further competitionsListedNot listed
Completing a training sessionNot listedListed
Fulfilling media commitmentsListedNot listed
Attending a medal ceremonyListedNot listed
Receiving necessary medical treatmentListedListed
Obtaining photo identificationListedListed
Locating a representativeListedListed
Locating an interpreterListedDCO review

Listed means a recognised reason for requesting a delay; approval is still required. Other reasonable circumstances may be considered by the DCO and must be documented.

Providing the sample

Observation arrangements

An authorised witness of the same gender directly observes sample provision in a private collection area.

Male athletes

The witness may stand beside the athlete, in a position that allows a clear, unobstructed view of sample provision.

Female athletes

The witness may be positioned in front of the athlete, maintaining a clear, unobstructed view of sample provision.

Minors

An adult representative is encouraged. During sample provision, a second collection staff member observes the DCO or chaperone, rather than the passing of the sample.

For a minor, the representative normally observes the DCO or chaperone. The representative may directly observe sample provision only if the minor requests it. Ask the DCO about arrangements or modifications you need.

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